Search results for "Pyruvic Acid"
showing 10 items of 33 documents
Heterogeneous Interactions between Gas-Phase Pyruvic Acid and Hydroxylated Silica Surfaces: A Combined Experimental and Theoretical Study
2019
The adsorption of gas-phase pyruvic acid (CH3COCOOH) on hydroxylated silica particles has been investigated at 296 K using transmission Fourier transform infrared (FTIR) spectroscopy and theoretical simulations. Under dry conditions (<1% relative humidity, RH), both the trans-cis (Tc) and trans-trans (Tt) pyruvic acid conformers are observed on the surface as well as the (hydrogen bonded) pyruvic acid dimer. The detailed surface interactions were further understood through ab initio molecular dynamics simulations. Under higher relative humidity conditions (above 10% RH), adsorbed water competes for surface adsorption sites. Adsorbed water is also observed to change the relative populations …
A novel intracellular mutualistic bacterium in the invasive ant Cardiocondyla obscurior.
2016
The evolution of eukaryotic organisms is often strongly influenced by microbial symbionts that confer novel traits to their hosts. Here we describe the intracellular Enterobacteriaceae symbiont of the invasive ant Cardiocondyla obscurior, 'Candidatus Westeberhardia cardiocondylae'. Upon metamorphosis, Westeberhardia is found in gut-associated bacteriomes that deteriorate following eclosion. Only queens maintain Westeberhardia in the ovarian nurse cells from where the symbionts are transmitted to late-stage oocytes during nurse cell depletion. Functional analyses of the streamlined genome of Westeberhardia (533 kb, 23.41% GC content) indicate that neither vitamins nor essential amino acids a…
Physiologische Wirkungen und Stoffwechsel dimerer Fettsäuren
1964
Die langfristige Verfutterung von 5% dimeren Fettsauren entspr. rund 1,5 g/kg Korpergewicht (bezogen auf das Endgewicht) ergab toxische Wirkungen: Wachstumsverzogerungen, verschlechterte Futterefficiency, Verschlechterung des Grundumsatzes, Senkung der Korpertemperatur, Storung der Leberfunktion und Ablagerung eines abnormen Pigments im RES und in Organzellen.
A comparison of the effectiveness of azelaic and pyruvic acid peels in the treatment of female adult acne: a randomized controlled trial
2020
AbstractChemical peels are widely used as therapeutic agents in dermatology and cosmetology. This study aims to explore the differences in the effectiveness of azelaic and pyruvic acid peels in the treatment of acne vulgaris. Eligibility criteria for participants were: female gender, 18–25 years of age, no dermatological treatment within the last 12 months and mild to moderate papulopustular acne. We treated 120 young women (with a mean age of 22 years old) with six peeling sessions at 2-week intervals. In the parallel clinical study design, one randomized group (n = 60, 50%) was treated using azelaic acid (AA), whereas the second group participated in pyruvic acid (PA) sessions. We evaluat…
Metabolomics of Human Amniotic Fluid and Maternal Plasma during Normal Pregnancy
2016
Metabolic profiles of amniotic fluid and maternal blood are sources of valuable information about fetus development and can be potentially useful in diagnosis of pregnancy disorders. In this study, we applied 1H NMR-based metabolic profiling to track metabolic changes occurring in amniotic fluid (AF) and plasma (PL) of healthy mothers over the course of pregnancy. AF and PL samples were collected in the 2nd (T2) and 3rd (T3) trimester, prolonged pregnancy (PP) until time of delivery (TD). A multivariate data analysis of both biofluids reviled a metabolic switch-like transition between 2nd and 3rd trimester, which was followed by metabolic stabilization throughout the rest of pregnancy proba…
Application of a MTT Assay for Screening Nutritional Factors in Growth Media of Primary Sponge Cell Culture
2004
Marine sponges (Porifera) are producers of the largest variety of bioactive compounds among benthic marine organisms. In vitro culture of marine sponge cells has been proposed for the sustainable production of these pharmacologically interesting compounds from marine sponges but with limited success. The development of a suitable growth medium is an essential prerequisite for sponge cells grown in vitro. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was adapted to screen for potential nutritional factors in formulating a growth medium for primary cell culture of Suberites domuncula. In 96-well plates, the effects of nutritional factors including glutamine, pyr…
A bioluminescence technique for quantitative and structure-associated imaging of pyruvate
2006
A novel bioluminescence assay has been developed for measuring pyruvate within sections of snap-frozen tissue in a quantitative manner as well as with a spatial resolution on a microscopical level. The assay was verified via HPLC and two independent photometric tests. The novel assay makes it possible to determine pyruvate concentrations in cryosections in the range of 0-5.0 micromol/g tissue (dry weight). Based on the analysis of samples of given pyruvate concentrations, the assay exhibits a recovery with a deviationor =15%. The minimal detectable amount was 0.02 pmol based on a 20 microm thick tissue section with an area of 1 cm(2). Combination of the already established imaging biolumine…
The Dissociation Constant of Pyruvic Acid: Determination by Spectrophotometric Measurements
1991
Aqueous solutions of sodium pyruvate/hydrochloric acid mixtures were studied with regard to changes in the intensity of optical absorption at 317 nm wavelength, caused by varying pH and temperature. The data were evaluated to determine the pyruvic acid apparent dissociation constant at infinite dilution. Its temperature dependence followed the relation ln Kod = −(0.55 ± 0.47) − (1538 ± 135)/T or, if previous results of Pedersen at two temperatures are included, In Kod = −(0.97 ± 0.35) − (1418 ± 102)T. The effective absorption coefficient for pyruvic acid and its anion differ because the former species is largely hydrated, whereas the latter is not. The known hydration constant and its tempe…
LACTATE DEHYDROGENASE ISOENZYMES IN THE NERVOUS TISSUE?V..
1965
The effect of time on physiological changes in eel Anguilla anguilla, induced by lindane.
1991
Abstract 1. 1. Eel were exposed to a sublethal concentration of lindane (0.335 ppm) for 6, 12, 24, 48, 72 and 96 hr. 2. 2. Concentrations of glycogen, glucose, lactate, pyruvate and lipids were determined in gill tissue after lindane exposure. 3. 3. Gill glycogen descreased and glucose levels increased at 6 hr of treatment, lactate and pyruvate concentration increased between 6 and 48 hr. Total lipid values decreased between 6 and 24 hr; thereafter, the levels increased up to 72 hr of exposure. 4. 4. Clear changes were found in all parameters tested in gill tissues. The observed effects of lindane on metabolism in fish are discussed in relation to acute stress syndrome.